Answer:
Extraction of genomic DNA
Explanation:
Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.
If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.
Answer:
A solar eclipse is Local
A lunar eclipse is global
Explanation:
h2o is water and c2o is carbon dioxide :)
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Decomposers also break down the bodies of dead organisms resulting in nitrogen being returned to the soil as ammonia. In some conditions denitrifying bacteria in the soil break down nitrates and return nitrogen to the air.
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