When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
Urinary bladder - superior mesenteric ganglion
Explanation:
The inferior mesenteric ganglion is located in the center of the abdomen where the inferior mesenteric artery begins. The axons of postganglionic neurons extend through the hypogastric plexus. These neurons then innervate the blood vessels of several organs like the rectum, urinary bladder, and distal colon, etc.
The postganglionic neurons from superior mesenteric ganglion innervate the blood vessels of the small intestine and proximal colon.
Prairie Grass is the organism that provides energy to all other organisms in the ecosystem.
Answer:
The World Ocean
Explanation:
The World Ocean is the largest existing ecosystem on our planet. Covering over 71% of the Earth's surface, it's a source of livelihood for over 3 billion people.
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<em><u>^Hope this helps, Hazel^</u></em></h2>
<span>A woman who regularly misses meals is the most likely to have a child suffering from lowered birth weight. This is because the woman is not receiving enough nutrients for both herself and the developing fetus, leading to undernourishment on the parts of both organisms and weights under what the recommended measures would be.</span>
