Answer:
POLYADENYLATION mechanism.
Explanation:
This is the mechanism of adding a poly(A)tail to a mRNA to protect the RNA.This tail is made up of adenosine monophosphate(AMP) compound, and therefore consists of only this purine base stretch.(adenine is purines).
Polyadenylation participate in production of mature mRNA for the process of translation in the cystosol, therefore ensuring stability.
However this tail shortened with time, and in extreme shortness the mRNA is degraded by enzymes in the cells.
Conclusively, the protection provided by the poly(A) tail
and production of mature mRNA ensured the stability of these molecules
<span>Simmon’s Citrate agar is used to determine an organism’s ability to use citrate as a sole carbon source.Simon’s citrate agar is a defined medium in which sodium citrate is the sole carbon source, and ammonium is the sole nitrogen source. Bromothymol blue (BTB) is included as a pH indicator
The medium is initially at pH 6.9, at which BTB is green; at a pH greater than 7.6 BTB turns a deep blue. The pH change is induced by CO2, which is given off as a by-product of citrate utilization. When it reacts with Na and H2O in the agar it raises the pH above 7.6
The organism must contain the enzyme citrase to degrade citrate
(citrase)
Citrate --------->CO2 + Na HCO + H2O
I
(blue color change)
Now, coming to how a citrate test may give false results. There are only 2 ways that can happen.
- Failure to incubate with loose caps may result in a false-negative results.
- Avoid using a large inoculum to streak the strand; an inoculum that is too heavy may result in a false-positive test. </span>
<span>The determining factor in how well a patient is able to compensate for loss of blood in usually related to how rapidly they are bleeding. The ability or the lack of ability to compensate for blood loss is controlled by a patient's cardiovascular system, but the rate of blood loss is key.</span>