Answer: B. Melting temperatures of primer should be between 55-80 degree Celsius.
Explanation:
Bacause the melting temperature controls the binding of the primers to your template DNA. At melting temperature 50% of the primer molecules are bound to their corresponding target sequence. If the difference in melting temperature between the two primers is too high, it might be difficult to find experimental conditions where both primers can bind to their target.
Yes, could most likely be a possible question that could have interesting results
Answer:
Interphase refers to all stages of the cell cycle other than mitosis. During interphase, cellular organelles double in number, the DNA replicates, and protein synthesis occurs. The chromosomes are not visible and the DNA appears as uncoiled chromatin.
Answer: Nitrogen is so vital because it is a major component of chlorophyll, the compound by which plants use sunlight energy to produce sugars from water and carbon dioxide (i.e., photosynthesis). It is also a major component of amino acids, the building blocks of proteins.
Explanation:
Answer:
Coelenterates are the first invertebrates to develop a true nervous system.
