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schepotkina [342]
2 years ago
5

What is Recombinant cloning

Biology
1 answer:
kumpel [21]2 years ago
4 0

Recombinant DNA technology / DNA cloning; gene cloning; cloning. A technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vectors that carry the DNA into a host cell.

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Describe the process of chromatographic separation. You need to include both the equilibrium model to predict retention times an
Kay [80]

Answer:Chromatography is actually a way of separating out a mixture of chemicals, which are in liquid or gas form, by letting them flow slowly past another substance, which is either a solid or a liquid. It consist of a stationary phase and a mobile phase.

Explanation: All chromatographic systems rely on the fact that a substance placed in contact with two unmixable phases, one movable phase and one stationary phase, will equilibrate between them. A selectivity (or separation) factor (α) is used to 'chemically' distinguish between sample components. It is usually measured as a ratio of the retention (capacity) factors (k) of the two peaks in question and can be visualized as the distance between the apices of the two peaks. reproducible fraction will partition into each phase, depending on the relative affinity of the substance for each phase. A substance which has affinity for the moving or mobile phase will be moved rapidly through the system. A material which has a stronger affinity for the stationary phase, on the other hand, will spend more time immobilized in that phase, and will take a longer time to pass through the system. Therefore, it will be separated from the first substance. By definition, chromatography is a separation technique in which a sample is equilibrated between a mobile and a stationary phase. A theoritical plate or tray is used to produces the best possible difference between the liquid and vapour phases in equilibrium with it

Chromatographic separations are best done with a small amount of analyte (substance to be separated during analysis), which keeps either phase from becoming saturated with analyte, so that the concentrations in the two phases are directly proportional. Overloading the column with sample causes one of the phases to become saturated, leading to a loss of column efficiency, and poorly shaped peak profiles.

The retention volume in chromatographic separation (Vr) is the volume of the mobile phase required to carry the solute through the column to elution, is related to the column flow (Fc) and the retention time (tr). Likewise, the volume of the mobile phase(Vm), is related to the flow and the time the void volume takes to pass through the column.

Band broadening using the kinetic model is a phenomenon that reduces the efficiency of the separation being carried out, leading to poor resolution and chromatographic performance. This is problematical in terms of both the quality of the separation obtained and the accuracy with which sample components can be quantified.The wider band results in a dilution effect that produces a decrease in peak height accompanied by a loss in sensitivity and resolution. The eddy dispersion, accounts for the source of band broadening related to any flow unevenness in the column.

4 0
3 years ago
Cual es la diferencia entre cigoto; embrión y feto
astra-53 [7]

Answer:

in spanish-Los términos embrión y feto se refieren al bebé en desarrollo dentro del útero de la madre. ... Un embrión se denomina feto a partir de la undécima semana de embarazo, que es la novena semana de desarrollo después de la fertilización del óvulo. Un cigoto es un organismo unicelular resultante de un óvulo fertilizado.

Explanation:

in english- The terms embryo and fetus both refer to the developing baby inside the mother's womb (uterus). ... An embryo is termed a fetus beginning in the 11th week of pregnancy, which is the 9th week of development after fertilization of the egg. A zygote is a single-celled organism resulting from a fertilized egg.

8 0
3 years ago
Which of the following is similar among all living organisms on Earth? social behavior, physical appearance, cellular function,
Mkey [24]
It is all of them. To be alive we all kind of have to have a face or body, we all need cells to help us with our day to day activities and we all need to communicate with other humans so that would be social behavior. 
3 0
3 years ago
Read 2 more answers
Specialized cells are grouped into structures known as
gregori [183]
Your answer should be tissues.
5 0
3 years ago
In a particular family, one parent has Type A blood, the other has Type B. They have four children. One has Type A, one has Type
AlladinOne [14]

Answer:

The correct answer is -

Parental genotypes: AO & BO

Children genotypes: AB, AO, BO, and OO

Explanation:

In the human blood group system, more than two types of alleles are present that lead to the existence of four blood groups in the human population.

Here, A and B alleles are co-dominant while the O allele is recessive to both A and B alleles in the ABO system.

The cross depicting the genotypes of children is

IA IO

IB    IAIB    IBIO

IO   IAIO   IOIO

In this cross, we get the same result as given in the question -

One parent has A and the other has B type blood while the children have all four types of blood groups One has Type A, one has Type B, one has Type AB, and the last has Type O. It is possible only when parents are heterozygous in their genotypes and not expressing codominance.

6 0
2 years ago
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