You have made a specific DNA probe that will bind to a key sequence on the DNA of Nesseria gonorrhoeae, allowing your company to
market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens. However, upon testing it out against a known Neisseria gonorrhoeae culture, you find that it does not work. Which of the following is a possible explanation for this negative result? A.You forgot to label the probe sequence with a reporter molecule.
B. You forget to digest the probe with restriction endonucleases
C. You forget to add the mRNA to the DNA probe sample.
D. You forgot to incubate the test at the 75C temperature required for hybridization.
A.You forgot to label the probe sequence with a reporter molecule.
Explanation:
B. You forget to digest the probe with restriction endonucleases: THERE IS NO NEED TO DIGEST DESIGNED PROBES WITH RESTRICTION ENDONUCLEASES.
C. You forget to add the mRNA to the DNA probe sample. THE DESIGNED PROBE IS TARGETING <em>Neisseria gonorrhoeae</em> DNA, NOT A TRANSCRIPT. SO, THERE IS NO NEED OF ADDING mRNA.
D. You forgot to incubate the test at the 75C temperature required for hybridization. THIS IS POSSIBLE TOO, BUT NOT ALL HIBRIDIZATIONS OCURR AT 75 C. IT DEPENDS ON THE PROBE SEQUENCE, SO, IN ORDER TO AFIRM THIS POINT YOU NEED TO KNOW THE PROBE LENGHT AND SEQUENCE.
