Two aligned sequences fo the same gene differ by four substitutions, all transitions, two more sequences for the same gene also
differ by found substitutions, two transitions and two transversals. which pair of sequences has probably been evolving independently longer and how do you know?
This is because transitions are interchangeable of two ringed purines A to G or of one ringed pyrimidine C to T they therefore involves bases of similar shapes.
Transition mutations are generated at higher frequency than transformation which is interchangeable of purines for pyrimidine bases which involves exchange of one ring and two ring structures.
Plantae, Archaebacteria, Eubacteria, and potentially fungi. I say potentially because I am not 100% certain if all organisms falling under the category of fungi contain cell walls.