Which of the chemical where are the chemical
Maintaining acidity- <span>In case the pH in the environment drops a lot (becomes very acidic), there is a flux of H ions from the cell's cytoplasm into the cell's vacuole thereby increasing acidity to required level and thus maintaining the </span><span>specific pH </span><span>in the cells internal environment as well.
Turgidity- </span><span>Vacuoles regulate the turgidity by regulating the amount of water inside the cell. </span>
cell has excessive water:<span> vacuole absorbs the water and then diffuses it out of the cell.</span>
cell lacks water<span>: water from the vacuole gets passed back into the cell thereby maintaining turgidity.
And here's a video </span>https://youtu.be/3tqjITi8S8s
Answer:
You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures. Describe the personal protective equipment that would be needed and three different culturing techniques that can be used to obtain organisms to produce pure cultures. State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated? What is the first step you would take if you detected contamination?
Personal Protective equipment needed; No special precautions are necessary when working with methicillin-resistant staphylococcus aureus, staphylococcus aureus is a bio-safety level
Three different culturing media; to include;
- Etest Method
- Disc Diffusion
- Latex Agglutination.
Properly enriched selective media is used.
The most accurate method for identifying Merthicillin-resistant staphylococcus is to test for the presence of mecA gene by PCR.
The best specific media is latex agglutination test and oxacillin salt agar screen.
How to determine if the culture was contaminated;
It can be spotted by the turbidity of the growth medium or the floating branching mycelia, bacteria contamination can be detected under 10× microscope within few days of contamination.
Step to take if contamination is detected;
Biological contamination is a serious issue and being able to correctly identify different types of contamination is an essential first step in ensuring the safety of your culture.
Explanation:
The literature on methicillin susceptible testing is extensive, and often conflicting in recommendations regarding the most important method for routine use. this is partly because the various studies of phenotypic methods have included different strains, which may differ significantly in heterogeneity and behave differently under particular test condition.
The key difference between age polyethism and reproductive division of labor in honey bees is their age and functions, younger bees work inside the honeycomb, they have several duties, such as feeding the larvae, cleaning, thermoregulate the inside and receiving the nectar from the older bees, through trophalaxy (mouth contact), those bees are in charge of the guard an cleaning of the outside, besides helping with the honeycomb ventilation.
It would be primary since the writer himself is getting the information from a direct source.
