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kow [346]
3 years ago
6

Which step in the PCR program would you have to change if your fragment would have been 10 times longer and how?

Biology
1 answer:
bekas [8.4K]3 years ago
7 0

Answer:

increasing extension time

Explanation:

The Polymerase Chain Reaction is a technique widely used in molecular biology laboratories to amplify target DNA regions. The standard steps of a PCR are as follow 1-denaturation, 2-annealing and 3-elongation/extension. These steps are repeated 15-40 times in order to exponentially amplify the linear DNA fragment. It is well known that longer extension times can be used as a strategy to increase the yield of longer PCR products. This is because the extension time depends on the synthesis rate of the DNA polymerase used in PCR technique and the length of the DNA fragment to be amplified.

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ANTONII [103]

Answer:

If there is low genetic diversity, she needs to introduce something into the system to increase the variation. I would suggest breeding the orange trees with other new orange trees to change the allele frequencies, which is called gene flow.

Likely the fungi are attacking them somewhere that they have decreased resistance, and if more alleles were introduced, there would probably be evolution towards a genotype that promotes defense against these fungi.

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3 years ago
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2 years ago
What is an un-duplicated chromosome called?
Oksana_A [137]
Unduplicated chromosomes are single chromosomes with a single line strand and one DNA molecule (if that's what you are looking for).
8 0
3 years ago
Read 2 more answers
What are the three parts of a DNA molecule
Contact [7]

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Explanation:

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krek1111 [17]

Answer:

Explanation:

I have been raised under an autthoritative parenting style, and my parents were both raised under an authoritarian style (it was more evident in my mother, because my father's mum died when he was 13). I think it was authoritative because they didn't let me do whatever I wanted, but they also didn't keep so much control on me, so I had certain level of freedom, as long as I didn't break anything xD

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