Question

Which step in the PCR program would you have to change if your fragment would have been 10 times longer and how?

Answer 1

Answer:

increasing extension time

Explanation:

The Polymerase Chain Reaction is a technique widely used in molecular biology laboratories to amplify target DNA regions. The standard steps of a PCR are as follow 1-denaturation, 2-annealing and 3-elongation/extension. These steps are repeated 15-40 times in order to exponentially amplify the linear DNA fragment. It is well known that longer extension times can be used as a strategy to increase the yield of longer PCR products. This is because the extension time depends on the synthesis rate of the DNA polymerase used in PCR technique and the length of the DNA fragment to be amplified.