Which step in the PCR program would you have to change if your fragment would have been 10 times longer and how?

Biology

1 answer:

bekas [8.4K]3 years ago

7 0

Answer:

increasing extension time

Explanation:

The Polymerase Chain Reaction is a technique widely used in molecular biology laboratories to amplify target DNA regions. The standard steps of a PCR are as follow 1-denaturation, 2-annealing and 3-elongation/extension. These steps are repeated 15-40 times in order to exponentially amplify the linear DNA fragment. It is well known that longer extension times can be used as a strategy to increase the yield of longer PCR products. This is because the extension time depends on the synthesis rate of the DNA polymerase used in PCR technique and the length of the DNA fragment to be amplified.

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Which of the following best represents the relationship of the concentration of hydrogen ions between water, vinegar, and ammoni

aalyn [17]

Answer: The second image best represents the relationship of the concentration of hydrogen ions between water, vinegar and ammonia.

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Mathematically,

$pH=-\log[H^+]$

The solutions which lie in the pH range of 0 - 6.9 are considered as acidic solutions and they have more hydrogen ion concentration.

The solution which has a pH value of 7 is considered as a neutral solution and this solution has same hydrogen and hydroxide ion concentration.

The solutions which lie in the pH range of 7.1 - 14 are considered as basic solutions and they have less hydrogen ion concentration.

For vinegar, the hydrogen ion concentration is very high.

For water, the hydrogen ion concentration will be equal to the hydroxide ion concentration.

For ammonia, the hydrogen ion concentration is very less.

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3 years ago

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The correct answer is: egg growth.

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3 years ago

Suppose two parents, a father with the genotype AaBbCcDdee and a mother with the genotype aabbCcDDEe, wanted to have children. A

Anon25 [30]

Answer:

Half of them

Explanation:

Each letter represent a character.

Capital letter represent dominance.

When you complete punnet's charters (one of each character) you will know the appearence percentage.

Letter A