Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
If the pancreas did not stop producing insulin and blood sugar levels did not dropped to normal levels so it causes a disease called hyperinsulinemia. This disease causes heart disease and cancer in the body. With increased levels of insulin makes the cells resistant to harmone which means there is no effects of harmone on the cell and the body didn't perform its functions properly. The increase in insulin levels increase the absorption of sugar from the blood and the person gets more weight which is not good for health.
Weathering breaks down and loosens the surface minerals of rock so they can be transported away by agents of erosion such as water, wind and ice. There are two types of weathering: mechanical and chemical.
Mycoplasmas differ from other bacteria in that they Lack a cell wall.
Mycoplasmas are different from bacteria as it can cause a wide range of infections and diseases. Mycoplasmas are phenotypically distinguished from other bacteria by their small size and complete absence of a cell wall. Lack of cell wall can be used to separate the mycoplasms from bacteria. It is a genus of bacteria that, like other members of the class Mollicutes, lack a cell wall around their cell membranes. Peptidoglycan is absent. This property makes them naturally resistant to antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic
Learn more about Mycoplasmas on:
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