Aero is the prefix for oxygen or air
Aerobic= needs air
Anaerobic=no air needed
Answer:
Cas9-sgRNA cannot align with targeted DNA sequence
Explanation:
Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.
Structurally, DNA and RNA are nearly identical. As mentioned earlier, however, there are three fundamental differences that account for the very different functions of the two molecules. RNA has a ribose sugar instead of a deoxyribose sugar like DNA. RNA nucleotides have a uracil base instead of thymine.
