Weathering plate movements and volcanoes destroyed many of the craters.
APEX
Answer:
The reason for the offspring to present these genotypes is that during the formation of the gametes, the alleles separate and are inherited independently, therefore they can generate several different phenotypic combinations.
Explanation:
In order for an offspring to present very different phenotypes, as shown in the question above, it is necessary that the two red griffins with blue eyes that were crossed are heterozygous. Thus it will be possible for the offspring to present a wide variety of phenotype, according to Mendel's second law.
Mendel's second law is called the Law of segregation. This law explains that the alleles (which determine the characteristics of individuals) are separated in the formation of gametes and inherited by the offspring of a cross independently, and can generate different combinations of phenotypes, when the parents of a cross are heterozygous.
I believe the term for an observable trait of an organism would be called or noted as the organism's phenotype.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Transcription, Translation, protein synthesis
