Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
The answer should be C.
An energy pyyramid shows how the energy "flows" from organism to organism as it goes up.
Answer: 6 is the wavelength has decreased.
Explanation:
In biology, cellular communication is a term used to determine distinct kinds of methods of communication among the living cellulites. Of these intercellular communication signifies the communication between the cells. In this kind of communication, membrane vesicle trafficking plays an essential role in both humans and animals.
On the other hand, intracellular communication signifies towards the communication of the organelles in order to sustain cellular homeostasis.
