Question

This is the general outline of Recombinant DNA Technology according to my teacher. Can you explain VI for me? It sounds a bit vague. Genuine answers only please, otherwise you get reported. Thank you
I. Cutting or cleavage of DNA by restriction enzymes (res)

Ii. Selection of an appropriate vector or vehicle which would propagate the recombinant

DNA (eg. Circular plasmid in bacteria with a foreign gene of interest)

Iii. Ligation (join together) of the gene of interest (eg. From animal) with the vector ( cut Bacterial plasmid)

Iv. Transfer of the recombinant plasmid into a host cell (that would carry out replication to

Make huge copies of the recombined plasmid)

V. Selection process to screen which cells actually contain the gene of interest

Vi. Sequencing of the gene to find out the primary structure of the protein​

Answer 1

Answer:

All plasmids we manufactured are free of animal-derived materials and can contain low levels of endotoxin (<100EU/mg, on request). This process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.