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White raven [17]
3 years ago
13

This is the general outline of Recombinant DNA Technology according to my teacher. Can you explain VI for me? It sounds a bit va

gue. Genuine answers only please, otherwise you get reported. Thank you
I. Cutting or cleavage of DNA by restriction enzymes (res)

Ii. Selection of an appropriate vector or vehicle which would propagate the recombinant

DNA (eg. Circular plasmid in bacteria with a foreign gene of interest)

Iii. Ligation (join together) of the gene of interest (eg. From animal) with the vector ( cut Bacterial plasmid)

Iv. Transfer of the recombinant plasmid into a host cell (that would carry out replication to

Make huge copies of the recombined plasmid)

V. Selection process to screen which cells actually contain the gene of interest

Vi. Sequencing of the gene to find out the primary structure of the protein​
Biology
1 answer:
natta225 [31]3 years ago
8 0

Answer:

All plasmids we manufactured are free of animal-derived materials and can contain low levels of endotoxin (<100EU/mg, on request). This process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.

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5. If an organism has 5 pairs of chromosomes and no crossing over occurs, how many different
Tom [10]

Answer:

In spite of the fact that he didn't have any acquaintance with it, Walther Flemming really noticed spermatozoa going through meiosis in 1882, yet he confused this cycle with mitosis. Regardless, Flemming saw that, dissimilar to during standard cell division, chromosomes happened two by two during spermatozoan improvement. This perception, continued in 1902 by Sutton's careful estimation of chromosomes in grasshopper sperm cell improvement, given conclusive insights that cell division in gametes was not simply customary mitosis. Sutton showed that the quantity of chromosomes was decreased in spermatozoan cell division, a cycle alluded to as reductive division. Because of this cycle, every gamete that Sutton noticed had one-a large portion of the hereditary data of the first cell.

Explanation:

3 0
3 years ago
A bacterium that you isolated from pond water appears to use light for energy. Based upon this information, you inoculate the or
FinnZ [79.3K]

The question is incomplete as it does not have the options which are:

  • there was no sulfur compound added to the medium, that could be used as an electron donor.
  • no oxygen was added to the medium so the organism died.
  • there is some inhibitory chemical that is preventing the growth of the bacterium.
  • you were using the wrong type of sunlight as the energy source for the bacterium.

Answer:

There was no sulfur compound added to the medium, that could be used as an electron donor.

Explanation:

In the given question, the bacteria which are found in the pond uses light energy to use carbon dioxide and form the glucose molecule. These bacteria are known as phototrophic bacteria.

The process of photosynthesis requires an electron donor and an electron acceptor to use molecule.

The organism when provided the light and carbon dioxide artificially in a culture, the bacteria were not able to grow. The reason for this could be accounted as that there was no electron donor found in the media like sulfur which could donate the electron during the chain reaction.

Thus, the selected option is correct.

4 0
3 years ago
To find the main idea of a piece of writing,you can employ the techniques in which you?​
Alika [10]

Answer:

The main idea in a given piece of reading is implied by the introductory paragraph, and, or several meaning sentences that are in subsequent paragraphs.

Explanation:

The main ideas in the a given piece of work, specifically reading is contained in the first paragraph of the piece of writing. The first paragraph can be used to imply the main ideas in a topic without necessarily revealing it. The first concepts, terms, illusion and characters mentioned, certainly we can deduce the main idea.

Several sentences in paragraphs that carry almost similar, related and interrelated facts, in most cases are very essential in explaining the main idea.  By looking at them in various paragraphs, one is able to find the sole message being communicated.

5 0
3 years ago
How are temperatures in a desert different from day to night?
MariettaO [177]
Very hot during the day, cold at night
Plz give me brainalist answer
4 0
2 years ago
Pls answer i need the answer​
podryga [215]

Answer:

i think it is 2 in 4 or 50 %

7 0
3 years ago
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