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White raven [17]
3 years ago
13

This is the general outline of Recombinant DNA Technology according to my teacher. Can you explain VI for me? It sounds a bit va

gue. Genuine answers only please, otherwise you get reported. Thank you
I. Cutting or cleavage of DNA by restriction enzymes (res)

Ii. Selection of an appropriate vector or vehicle which would propagate the recombinant

DNA (eg. Circular plasmid in bacteria with a foreign gene of interest)

Iii. Ligation (join together) of the gene of interest (eg. From animal) with the vector ( cut Bacterial plasmid)

Iv. Transfer of the recombinant plasmid into a host cell (that would carry out replication to

Make huge copies of the recombined plasmid)

V. Selection process to screen which cells actually contain the gene of interest

Vi. Sequencing of the gene to find out the primary structure of the protein​
Biology
1 answer:
natta225 [31]3 years ago
8 0

Answer:

All plasmids we manufactured are free of animal-derived materials and can contain low levels of endotoxin (<100EU/mg, on request). This process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.

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Which of the following is NOT a membrane-disrupting toxin?
Vaselesa [24]

Answer:

Answer is A-B toxin.

Explanation:

A membrane-disrupting toxin is toxin that affect the cell membrane. The effect of its secretion  could be by interrupting the phospholipid layer or through pores formation on the membrane.

Membrane- disrupting toxins are regarded as  exotoxins. Examples are leukocidin and hemolysin which their effects cause leakages of the cytoplasmic content and lysis of the cell, through the formation of pores on the cell membrane.

The A-B toxin are produced by the proteins of pathogenic organisms such as the bacteria. Example is botulinum toxin.

3 0
3 years ago
Guysss please I need your help!!!
Katen [24]

Answer:

Symptoms

Signs and symptoms of hemophilia vary, depending on your level of clotting factors. If your clotting-factor level is mildly reduced, you may bleed only after surgery or trauma. If your deficiency is severe, you may experience spontaneous bleeding.

Signs and symptoms of spontaneous bleeding include:

  • Unexplained and excessive bleeding from cuts or injuries, or after surgery or dental work
  • Many large or deep bruises
  • Unusual bleeding after vaccinations
  • Pain, swelling or tightness in your joints
  • Blood in your urine or stool
  • Nosebleeds without a known cause
  • In infants, unexplained irritability

Bleeding into the brain

A simple bump on the head can cause bleeding into the brain for some people who have severe hemophilia. This rarely happens, but it's one of the most serious complications that can occur. Signs and symptoms include:

  • Painful, prolonged headache
  • Repeated vomiting
  • Sleepiness or lethargy
  • Double vision
  • Sudden weakness or clumsiness
  • Convulsions or seizures

Causes

When you bleed, your body normally pools blood cells together to form a clot to stop the bleeding. The clotting process is encouraged by certain blood particles. Hemophilia occurs when you have a deficiency in one of these clotting factors.

There are several types of hemophilia, and most forms are inherited. However, about 30% of people with hemophilia have no family history of the disorder. In these people, an unexpected change occurs in one of the genes associated with hemophilia.

Acquired hemophilia is a rare variety of the condition that occurs when a person's immune system attacks clotting factors in the blood. It can be associated with:

  • Pregnancy
  • Autoimmune conditions
  • Cancer
  • Multiple sclerosis

Treatment

Several different types of clotting factors are associated with different varieties of hemophilia. The main treatment for severe hemophilia involves receiving replacement of the specific clotting factor that you need through a tube placed in a vein.

This replacement therapy can be given to combat a bleeding episode that's in progress. It can also be administered on a regular schedule at home to help prevent bleeding episodes. Some people receive continuous replacement therapy.

Replacement clotting factor can be made from donated blood. Similar products, called recombinant clotting factors, are manufactured in a laboratory and aren't made from human blood.

Other therapies may include:

  • Desmopressin. In some forms of mild hemophilia, this hormone can stimulate your body to release more clotting factor. It can be injected slowly into a vein or provided as a nasal spray.
  • Clot-preserving medications. These medications help prevent clots from breaking down.
  • Fibrin sealants. These medications can be applied directly to wound sites to promote clotting and healing. Fibrin sealants are especially useful in dental therapy.
  • Physical therapy. It can ease signs and symptoms if internal bleeding has damaged your joints. If internal bleeding has caused severe damage, you may need surgery.
  • First aid for minor cuts. Using pressure and a bandage will generally take care of the bleeding. For small areas of bleeding beneath the skin, use an ice pack. Ice pops can be used to slow down minor bleeding in the mouth.
  • Vaccinations. Although blood products are screened, it's still possible for people who rely on them to contract diseases. If you have hemophilia, consider receiving immunization against hepatitis A and B
6 0
3 years ago
I need help with this
Nadusha1986 [10]

Answer:

asffgsd

Explanation:

6 0
3 years ago
Where does the fetus normally develop?
mart [117]

Answer:

in the womb !!

Explanation:

hAve a good night

7 0
3 years ago
Read 2 more answers
If two erminette chickens were crossed, what is the probability that: a. They would have a black chick? ________% b. They would
lisabon 2012 [21]

Answer:

a. They would have a black chick? 25%

b. They would have a white chick? 25%

8 0
3 years ago
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