Higher-order folding involves association of the DNA with a nuclear scaffold, which contains large amounts of Histone H1 and Topoisomerase II.
<h3>What is Histone?</h3>
Histones are highly basic proteins abundant in lysine and arginine residues that provides structural support for a chromosome. They are found in eukaryotic cell nuclei. They act as spools around which DNA winds, which gives the chromosome a more compact shape, to create structural units called nucleosomes. Nucleosomes in turn are wrapped as 30-nanometer fibers that form tightly packed chromatin. There are five types of histones namely H2A, H2B, H3, H4 and H1 linker histone. Within a nucleosome, they exist as two dimers of (H2A-H2B) and a complex of (H32-H42) eventually forming an octamer.
<h3>What is Topoisomerases?</h3>
Topoisomerases are nuclear enzymes which play essential roles in DNA replication, transcription, chromosome segregation, and recombination.
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identifying for components for 1 trial of this investigation is 185
Metals are malleable
Metals are ductile
Metals are good heat and electricity conductors
Metals lose electrons in reactions.
The question is incomplete as it does not have the options which are:
The empty vector would migrate the same distance as the cloned vector.
It is impossible to know without knowing the identity of the gene of interest.
The empty vector would migrate farther down than the cloned vector.
The cloned vector would migrate farther down than the cloned vector.
Answer:
The empty vector would migrate farther down than the cloned vector.
Explanation:
In recombinant technology, an empty vector is considered a vector which is present without the gene of interest and thus is of small size and the cloned vector is the vector which has a gene of interest along with the usual sequence.
The cloned vector is larger compared to the empty vector as the cloned vector has a gene of interest whereas the empty vector does not have.
In-gel electrophoresis technique, the DNA samples are run and the size of the fragment is known according to the ladder sequence which is used as a reference and have the band size in increasing order from lower side to upper side.
When the vectors sun on the gel, the small size gene will move faster and to the farthest distance compared to the larger DNA that is empty vector will cover the maximum distance while the cloned vector will cover the less distance.
Thus, the selected option is correct.
Can you add a picture so I can see what you mean?
