The boiling point of tap water is 100 degrees C (not Farenheit).With a tablespoon of salt, it would be slightly warmer than that.
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the respiration rate will increase as temperature increases. An increase in temperature will minimize the amount of dissolved oxygen in the water and increase the metabolic rate of the goldfish, thus causing the goldfish's respiration rate to increase (“Hot waters make it hard for fish to breathe”).
Fixation answer : hope this helps
1) Store the cells DNA
2) Control cell division and cell growth