1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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Women's speech reflects women's traditional lesser power in American society.
<h3><u>Explanation:
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According to Lakoff’s book, the way people communicate holds a clear distinction about who holds the power. The language used by both genders depicts gender inequalities and also leads to a lack of women’s power in areas that involves women. In addition, Lakoff states that the language women use to communicate indicate their inferiority and weakness.
She recognized gestures such as hedging, super polite forms, tag questions and many more in women’s language. While this is notable there has been debate regarding gender inequalities arising from language and communication.
Geert Hofstede is a <span>Dutch </span>psychologist and anthropologists who has studied cultures all over the world and developed the framework for cross-cultural communication. According to Geert Hofstede, deep culture is <span>the collective programming of the mind that distinguishes the members of one human group from another.
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Answer:
Homophobia
Explanation:
According to my research on the Stonewall raid, I can say that based on the information provided within the question to the victims of these raids they considered it as a clear case of Homophobia. This is a term that is defined as hatred towards anyone that feels an attraction to their same-sex gender.
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Answer:
The South African government is divided into three parts: the Executive (the Cabinet), the Legislature (Parliament), and Judiciary (the courts).
Explanation:
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