c. Challenge: You are working with a neuronal cell line isolated from a human brain tumor. It appears to have a neuronal phenoty
pe when grown in culture on laminin coated dishes but appears to have more of a typical round, small (3 to 5 micrometer) phenotype when grown on a standard, collagen-only coated dish or a poly-lysine coated (positive charge only - no ECM component) dish. Under all 3 cases the cells attach to the substrate. You suspect that the reason the neuronal phenotype is supported by the laminin coating is due to the ability of laminin, but not collagen or poly-lysine, to bind to the integrin receptors RGD binding domain thus triggering a molecular cascade that leads to the expression of the neuronal phenotype. How might you test this hypothesis
This process can be performed using immunofluorescence analysis via antibodies.
Explanation:
<u>To test for the hypothesis</u>
To test for the hypothesis we have to analyze whether the neuronal phenotype is due to the binding of laminin to integrins, and this will enable us to determine which integrins are abundantly expressed on the neuronal cell membrane. This process can be performed using immunofluorescence analysis via antibodies
Finally when no differentiation markers are expressed in the laminin coated plates with mutant cells we can now say that it expresses integrins
Answer: Errors that occur during DNA replication lead to mutations, which represent permanent, heritable changes in genetic material. These errors can pass from parent cell to daughter cells and affect many generations of cells.
Microbes are everywhere in the biosphere, and their presence invariably affects the environment that they are growing in. The effects of microorganisms on their environment can be beneficial or harmful or inapparent with regard to human measure or observation.
to determine whether traits can be inherited independently
Explanation:
the use of dihybrid crosses allowed him to discover the law of independent assortment / discovering that each locus had 2 alleles which segregate during the creation of gametes.