Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
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Answer:
<em>Plants give out carbon dioxide not only at night but during the day too. It happens because of the process of respiration in which plants take in oxygen and give out carbon dioxide. As soon as the sun rises another process called photosynthesis starts, in which carbon dioxide is taken in and oxygen is given out</em>
Depends, if the biotic factor is a chipmunk or something of that case, its natural habitat is a rock, which is biotic. but...if the animal is something like a deer they don't need abiotic factors. its your chose to chose but I'm leaning more twords false because not ALL biotic factors cant go without an abiotic factor
