Seismometers and Seismographs
SBP is a serious complication in patients with cirrhosis with high mortality rates (20–40%).
Patients at risk of developing SBP can be categorized in three groups firstly patients with active variceal bleeding patients with ascitic fluid protein <10 g/dl secondly those with a prior episode of SBP.
The most common bacteria causing SBP are gram-negative Escherichia coli and Klebsiella pneumoniae and gram-positive Streptococcus pneumoniae usually only a single organism is involved. Spontaneous bacterial peritonitis (SBP), an infection of ascitic fluid without a definitive intra-abdominal source that can be surgically treated is a common complication in patients with cirrhosis and ascites. Spontaneous bacterial peritonitis (SBP),is treated with a 10 to 14 day course of antibiotics .
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Responsiveness to the environment;
growth and change;
ability to reproduce;
have a metabolism and breathe;
maintain homeostasis;
being made of cells; and.
passing traits onto offspring.
Answer:
tetracycline
Explanation:
When the DNA insert is ligated in Pstl site, the ampicillin gene is disrupted while <em>tetracycline gene is still intact</em>.
- If the bacterial colony is growth in a medium with only ampicillin, only the cells that contains the plasmid with the insert will grow.
- If the bacterial colony is growth in a medium with only tetracycline, the cells which have incorporated the plasmid will survive. We cannot distinguish between cells with plasmid + insert and plasmid alone.
- If the bacterial colony is growth in a plate with ampicillin + tetracyclin, only the cells with the plasmid without the insert will grow.
Therefore, to ensure that the cells of a bacterial colony contains the plasmid, they have to be seeded in a plate with tetracycline.