In the 1950s, Christian Anfinsen demonstrated the renaturation of the protein ribonuclease (RNase) in vitro. After reduction and
the addition of urea, the protein was in an unfolded state. After removing the urea and then the reducing agent, the protein oxidized and refolded, with greater than 90 % activity. If reducing agent removal occurs before removing the urea, the protein showed less than 5 % activity. Why would the protein not refold correctly if the urea were removed after the reducing agent was removed? (In other words, what would happen if the urea were removed after oxidation?) Choose the best answer. a. Urea would participate in weak bonding interactions with RNase, preventing oxidation of Cys.
b. The protein would not fully unfold (denature).
c. Disulfide bonds are not positioned correctly unless weak bonding interactions are present.
d. Contaminants in the RNase preparation would form covalent bonds with the protein, preventing reactivation.
Because poor holding communication appropriately place the - SH deposits to frame disulfide spans while expelling the diminishing operator (permitting oxidation) while evacuating the urea. prior to the evacuation of urea, oxidation happens so the cysteine buildups may not be found accurately and the disulfide spans that structure in an inappropriate spots bringing about inert protein
Yes it does for example the science room has the materials to be safe during school.No because there is still the covi going on and some students aren't taking it seriously.One concerne that I have is that if they need help with their studies their should be tutoring
Explanation: Bacteria are infinitesimal organisms that have single cells that grow in different atmospheres. They have an easily understood inside arrangement. Their cells are normally surrounded by two shielding coverings which are an external cell continuous vertical structure and a cell pliable sheet-like structure acting as a boundary inside. However, some bacteria do have a third shielding sheet furthest from the center named the capsule.
