A student is investigating how substrate concentration affects the rate of anenzyme-catalyzed reaction. Which threevariables s
hould be kept constant in the setup of thestudent’s investigation? Select all three variablesA. temperature of the reactionB. amount of enzymeC. amount of substrateD. amount of productE. pH of the reaction
Temperature should be kept constant because enzymes operate at different activity rates depending on the temperature. The same goes for the pH as well with different enzymes having different optimal pH levels.
The amount of enzyme should be kept constant as well because different concentrations of enzymes can determine how fast the reaction will occur.
Essentially anything that could affect enzyme activity apart from substrate concentration should be kept constant.
One significant difference that I noticed was the color of the water. In the control aquarium, the color of the water at 30 days was the same as it was on the 1st day. In the experimental aquarium, the water had a green tint on the 30th day, but on the first day it was clear.
