Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
Hello,
The prpuse of protiens is to help keep your body healthy and also it is to Repair anything broken torn or Fractred or Sprained
Hope this Helps
Plz mark me as Brainsit or Verifed Answer thanks!!!!
Answer:
See explanation.
Explanation:
Hello.
In this case we can for instance take into account the following mixtures:
- Orange juice.
- Tea.
- A soda.
- Salty water
- Coffee.
Thus, since the orange juice is a suspension as long as it contains different sizes of orange pulp particles that cannot be dissolved mostly because of its organic nature.
Tea can be a solution when we place a tea bag in a cup with warm water since just soluble components of tea are successfully dissolved in the water. Moreover, homemade tea based on some plants are fruits may behave as a suspension due to the oraginic particles that are not dissolved in the water yet suspended.
A soda is a solution of some water-soluble compounds, gaseous soluble carbon dioxide and water which are homogeneously distributed in the solution.
Salty water can be a solution if you put some salt in warm or just cold water and wait until it dissolves completely.
Coffee can be also a solution or suspension depending on how it is made, which type of coffee is used and the amount of coffee added to the hot water.
Best regards.
Helo i think the answer to this question is trait
Answer:
For your body to cool down one method is to sweat.
Explanation: Your sweat glands release sweat, which cools your skin as it evaporates. This helps lower your internal temperature