Your answer is D lives in the same region as others closely related species. Because it wouldn't be C cause that's not isolation it would be B cause there not plants and its not A because it is reproductive but its not isolation.
C. Both investigations have benefits and limitations and should be examined together to get the most reliable result
Genotype of heterozygous purple plant is: Pp
Cross = Pp × Pp
Offspring: = PP, Pp, Pp, pp
Pp, PP and pp is your answer
:D hope it helps