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Svetach [21]
3 years ago
14

A patient comes to you with a "PUS" sample and asks you to isolate & identify the Staphylococcus aureus form this sample the

n how you can isolate and identify the Staphylococcus aureus from the "PUS" sample. Kindly explain in sequence step by step.
Biology
1 answer:
Irina-Kira [14]3 years ago
7 0

Answer: Staphylococcus aureus can be isolated from the pus sample through the following steps:

--> Step 1: Describe the appearance of the specimen

--> Step 2: Examine the specimen microscopically

--> Step 3: Culture and report your observations.

--> Step 4: Biochemical testing

Explanation: Staphylococcus aureus are gram positive cocci bacteria that can be isolated from pus samples.Pus samples, which may come from wounds, abscess, burns or sinuses, are the type of samples which do not contain microorganisms and should not contain contaminants providing an aseptic collection technique and sterile container are used.

Staphylococcus aureus can be isolated from the pus sample through the following steps:

--> Step 1: White, yellow, brown, red, or black granules of varying size, shape, and consistency may be found in pus draining from

sinuses while describing the appearance of the pus sample.

--> Step 2: Here the specimen is examined microscopically after being stained with a staining technique called Gram staining. This step is done before culture to know the type of medium to use after gram positive cocci is microscopically seen.

--> Step 3: there are different cultures media that can be used to inoculate and culture a pus specimen. Since we are to isolate staphylococcus aureus, a selective and differential media known as Mannitol salt agar (MSA) is used. The presence of yellow colonies with yellow zone after incubation at 37°C indicate the presence of Staphylococcus aureus or species. There for the last confirmation test known as biochemical test is done.

--> Biochemical test: This is a chemical test that is done to identify a particular bacteria. For staphylococcus aureus, COAGULASE test is used to identify staphylococcus aureus which produces COAGULASE enzyme. A positive COAGULASE test is an indication of the presence of staphylococcus aureus as the bacterium isolate of the specimen.

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One biological factor that all living things are subject to suffer from is osmotic pressure. Halophilic microorganisms have developed mechanisms to adapt to saline environments where osmotic pressure acts with great intensity on individuals. These bacteria change the chemical composition of their membranes and also accumulate osmoprotective compounds in their cytoplasm to compensate for osmotic stress.

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6 0
3 years ago
In RNA, the amount of adenine present is always equal to what?
Ira Lisetskai [31]
The amount<span> of guanine </span>always equals the amount of cytosine<span>.

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5 0
3 years ago
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When a plant grows toward or away from a stimulus, it’s called a tropism.
harina [27]

Explanation:

Phototropism- a response to <u>light stimulus</u> that directs the stem to grow toward the light and roots to grow away from it.

ii. Gravitropism- Stems and leaves grow away from the force of <u>gravity</u> while roots grow toward it.

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3 years ago
Which statement correctly compares mass and weight?
hram777 [196]

Answer:

D. Weight varies with location, but mass does not vary

Explanation:

Weight can be defined as the force acting on a body or an object as a result of gravity.

Mathematically, the weight of an object is given by the formula;

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Mass can be defined as a measure of the amount of matter an object or a body comprises of. The standard unit of measurement of the mass of an object or a body is kilograms.

Irrespective of the location of an object or a body at a given moment in time, the mass (amount of matter that they're made up of) is constant. This ultimately implies that, whether you're in the moon, space, earth or any other place, your mass remains the same (constant).

<em>Hence, the statement that correctly compares mass and weight is that, weight varies with location, but mass does not vary. This is simply because acceleration due to gravity changes with location i.e its value varies with the planets.</em>

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