1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
lubasha [3.4K]
2 years ago
13

In a separate line of experiments, you are studying a protein that you suspect shuttles between the nucleus and the cytoplasm. H

owever, when you localize a GFP-tagged fusion protein, it appears to be only in the nucleus. To test whether it shuttles, you set up a heterokaryon assay. You take a cell expressing the GFP fusion protein and partially fuse that cell with another that does not express the GFP protein. The result is a heterokaryon that has a single cytoplasm but two separate nuclei. You treat the heterokaryon with the translation inhibitor cycloheximide for several hours and then examine the localization of the GFP protein. What do you predict will be observed if the GFP protein shuttles between the nucleus and the cytoplasm
Biology
1 answer:
Vanyuwa [196]2 years ago
4 0

Answer:

Following are the responses to these question:

Explanation:

The heterokaryons were cells of diverse traditions or more nuclei. A heterokaryon cell with network cells (donor and receiver nuclei) sharing a common costly to treat is produced whenever a node containing GFP-tagged protein (donor cell) is merged to a cell non express the GFP-tagged proteins (recipient cell). Unless the intracellular scram nuclear exists, GFP must leave the GFP atoms, be shipped to the cytosol, and be exported to a nucleus with emission of GFP protein (recipient nucleus). It is obtaining nuclear has been beginning to release the GFP protein.

Unless the GFP protein doesn't display a nucleus costly to treat, this is not distributed across time in the metal target. By either sole dissemination through nuclear pores or receptor-mediated routes, protein yelling from nuclear to emotional exhaustion can occur. That GFP proteins are shown in a nucleus only suggests a path via a transmitter. For chloroplast, though, protein disperses via nuclear envelope. It is not the case. It should have nuclear foreign trade signals when ferrying between the nucleus and cytoplasm.

Cycloheximide is indeed a medicine that stops protein expression without elongating. For heterokaryon cells, it inhibits fresh protein synthesis. It makes visualization of a nanoparticle cell of only old pre-existing molecules (before cycloheximide diagnosis). That post GFP substance is a shuttling shielding substance that passes seen between the nucleus and the cytoplasm and the receiver nucleus. Whenever the levels of the shuttle were high, the GFP protein expression is shown both by the sender and receiver nucleus. The recipient nucleus will not be left without any schlepping protein. Thus, the donor nucleus can only be used.

You might be interested in
Explain the main functions of the roots
Vesnalui [34]

Answer:

to anchor the plant to the ground. equalizing osmosis in the plant and feeding water up through the roots.

4 0
2 years ago
To test how fertilizer affects tomato plants, a farmer divided a field of young tomato plants into 10 plots of equal size. The f
Lapatulllka [165]

Answer:

c

Explanation:

8 0
3 years ago
Read 2 more answers
Livestock farming creates large amount of animal waste. How would this affect the nitrogen cycle?
777dan777 [17]

the nitrogen cycle involves decompisition. and the waste from the animals is then turned into nitrogen.

7 0
3 years ago
As you completed this assignment and reviewed the lectures (see the socioecological model lecture and diagram provided), you rea
Jlenok [28]

Answer:

factors that influenced daily food choices:

  • biological determinants
  • economical determinants
  • physiological determinants
  • physical determinants
  • social determinants
  • behavioral determinants

Explanation:

Social -ecological system is the set of different factors which influence our living styles, our dietary habits, our dress choice etc. on a daily basis. In today's world, everything act is done by people are only based on its popularity or followed by trends. Similarly, our daily food choices are also influenced by the trends and our surroundings in which we live. There is the impact of multiple factors on our daily food choices.

  1. Behavioral factors: behavioral factor is the most important factor which have a huge impact on our daily food choice. An individual health condition, behavior, attitude, work routine majorly influence the food choices. An individual personal preferences, likes and dislikes also influenced his food choices.
  2. Biological factors: these factors can influence individually as well as globally. Hunger, nutrient requirements, condition of safe food, health conditions are the major biological factors which play major role, when it comes to choose daily food. These factors can affect the global food preferences, for example in the pandemic of convid-19 food choice were similar of every individual globally.
  3. Economic factors: availability of food, cost of food, balancing of needs and demands also impacts our daily food choices. If cost are too high of basic food and the  availability is only limited to certain people, it automatically impacts on the food choice of an individual.
  4. Social Factor: when we talk about social factors these are the group of different small factors like culture, work environment, school, colleges, religious preferences, friends circles, demographic conditions etc. our daily food choices are mostly based on the social factors because our likes and dislikes, meal patterns, our preference of food (cooked, raw, packages, cooking style etc.)  Everything based on the impact of the surroundings. In schools and colleges, students are bound to eat what is available or what their friends prefer to eat. Religious backgrounds always choose as per their rulings. Traditional foods, cultural heritages also an impactful factor for our daily food choices.
  5. Physiological factors: mental health condition are also one of the important factor which can not be neglected when we choose our food to eat. A healthy individuals preferences will always be different from a sick person.

Social-ecological model can help us with our daily food choices by giving us the  knowledge of the factors and its healthy impact on our food choices which can lead us to a healthy life and prosperous environment. Smart choice not only make us health but also make the environment healthy for our lives.

7 0
2 years ago
During the replication of DNA, where are bonds broken?
Nady [450]
During DNA replication, the hydrogen bonds must be broken between the complementary nitrogenous bases in the DNA double helix
7 0
3 years ago
Other questions:
  • On an expedition to a barren land that had witnessed volcanic activity, geologists noticed heavy lichen growth on some of the ha
    12·2 answers
  • Pee wee herman's shoulders and waist are almost exactly the same width, so he is an example of which body type?
    7·1 answer
  • 3. Is a bone a living structure, or merely a deposit of mineral compounds?
    6·1 answer
  • Which process releases energy instead of using energy?
    12·2 answers
  • Joe is conducting an experiment to determine which liquid will cause bean plants to grow faster. He watered the plants with equa
    5·1 answer
  • Cellulose differs from starch in that?
    15·1 answer
  • How are<br> proteins<br> involved in<br> DNA<br> replication?
    15·1 answer
  • Unlike DNA, RNA contains
    11·1 answer
  • Which organelle controls when and what proteins are made? *
    8·1 answer
  • What Cell is squarish Shape at<br> Options Plant Cell<br> Animal Cell
    10·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!