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lubasha [3.4K]
2 years ago
13

In a separate line of experiments, you are studying a protein that you suspect shuttles between the nucleus and the cytoplasm. H

owever, when you localize a GFP-tagged fusion protein, it appears to be only in the nucleus. To test whether it shuttles, you set up a heterokaryon assay. You take a cell expressing the GFP fusion protein and partially fuse that cell with another that does not express the GFP protein. The result is a heterokaryon that has a single cytoplasm but two separate nuclei. You treat the heterokaryon with the translation inhibitor cycloheximide for several hours and then examine the localization of the GFP protein. What do you predict will be observed if the GFP protein shuttles between the nucleus and the cytoplasm
Biology
1 answer:
Vanyuwa [196]2 years ago
4 0

Answer:

Following are the responses to these question:

Explanation:

The heterokaryons were cells of diverse traditions or more nuclei. A heterokaryon cell with network cells (donor and receiver nuclei) sharing a common costly to treat is produced whenever a node containing GFP-tagged protein (donor cell) is merged to a cell non express the GFP-tagged proteins (recipient cell). Unless the intracellular scram nuclear exists, GFP must leave the GFP atoms, be shipped to the cytosol, and be exported to a nucleus with emission of GFP protein (recipient nucleus). It is obtaining nuclear has been beginning to release the GFP protein.

Unless the GFP protein doesn't display a nucleus costly to treat, this is not distributed across time in the metal target. By either sole dissemination through nuclear pores or receptor-mediated routes, protein yelling from nuclear to emotional exhaustion can occur. That GFP proteins are shown in a nucleus only suggests a path via a transmitter. For chloroplast, though, protein disperses via nuclear envelope. It is not the case. It should have nuclear foreign trade signals when ferrying between the nucleus and cytoplasm.

Cycloheximide is indeed a medicine that stops protein expression without elongating. For heterokaryon cells, it inhibits fresh protein synthesis. It makes visualization of a nanoparticle cell of only old pre-existing molecules (before cycloheximide diagnosis). That post GFP substance is a shuttling shielding substance that passes seen between the nucleus and the cytoplasm and the receiver nucleus. Whenever the levels of the shuttle were high, the GFP protein expression is shown both by the sender and receiver nucleus. The recipient nucleus will not be left without any schlepping protein. Thus, the donor nucleus can only be used.

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Plantlike photosynthesis that releases O2 occurs in
Ray Of Light [21]

Answer:

Cyanobacteria. (Ans. A)

Explanation:

Cyanobacteria are prokaryotes, are a phylum of bacteria. They are also called as blue-green algae and obtain their energy through the process of photosynthesis. They are commonly found in fresh water and terrestrial environment such as land, in rocks even in animal cells (fur).

Cyanobacteria are photosynthetic (oxygenic) bacteria. They harvest the energy of sun, absorb carbon dioxide and release O2. Cyanobacteria also contain chlorophyll such as plants and algae and convert carbon dioxide to sugar through the process of carbon fixation.

Cyanobacteria lack nucleus and other organelles like found in algae and plants. Instead of these they have double outer cell membrane and folded inner thylakoid member which are used in the process of photosynthesis.

5 0
3 years ago
In cats, curled ears result from an allele, Cu, that is dominant over an allele cu, for normal ears. Black color results from an
just olya [345]

Answer:

Explanation:

Cross : ggCuCu X GGcucu --------Parents

F1 progeny : GgCucu

Two of the F1 cats mate :

Cross : GgCucu X GgCucu

Gametes :

Gg X Gg = GG, Gg, Gg, gg

Cucu X Cucu = CuCu, Cucu, Cucu, cucu

1/4 GG X 1/4 CuCu = 1/16 GGCuCu (black - curled ears)

1/4 GG X 2/4 Cucu = 2/16 GGCucu (black - curled ears)

1/4 GG X 1/4 cucu = 1/16 GGcucu (black - normal ears)

2/4 Gg X 1/4 CuCu = 2/16 GgCuCu (black - curled ears)

2/4 Gg X 2/4 Cucu = 4/16 GgCucu (black - curled ears)

2/4 Gg X 1/4 cucu = 2/16 Ggcucu (black - normal ears)

1/4 gg X 1/4 CuCu = 1/16 ggCuCu (gray - curled ears)

1/4 gg X 2/4 Cucu = 2/16 ggCucu (gray - curled ears)

1/4 gg X 1/4 cucu = 1/16 ggcucu (gray - normal ears)

Answer : (i). 9/16 black cats, curled ears; 3/16 black cats, normal ears; 3/16 gray cats, curled ears; and 1/16 gray cats, normal ears.

An F1 cat mates with a stray cat that is gray and possesses normal ears :

Cross : GgCucu X ggcucu

Gametes :

Gg X gg = Gg, Gg, gg, gg

Cucu X cucu = Cucu, Cucu, cucu, cucu

2/4 Gg X 2/4 Cucu = 4/16 GgCucu (black - curled ears)

2/4 Gg X 2/4 cucu = 4/16 Ggcucu (black - normal ears)

2/4 gg X 2/4 Cucu = 4/16 ggCucu (gray - curled ears)

2/4 gg X 2/4 cucu = 4/16 ggcucu (gray - normal ears)

Answer : (i). 1/4 black cats, curled ears; 1/4 black cats, normal ears; 1/4 gray cats, curled ears; 1/4 gray cats, normal ears.

6 0
3 years ago
Mutated sequence:AUGUAAGUUUGGC
Eddi Din [679]

No, because it still codes for valine in this case, but if another nucleotide was changed such as GCA it would code for alanine instead.

8 0
2 years ago
Which layer of the human energy field is most closely associated with the body's blueprint of physical health and is found close
Marina CMI [18]

The etheric  body

The etheric body is the layer of the human energy field that is most closely associated with the body's blueprint of physical health and is found closest to the physical body.

The etheric body is the first layer of the human energy field that is found to be in immediate contact with the physical body. The ether body is the energy system for the physical body as it sustains it, joins it with higher bodies and functions as the matrix for physical metabolic activities.


4 0
3 years ago
One kind evidence that supports Wagner's hypothesis is that
anyanavicka [17]

Answer:

Wegener used evidence of climate change to support his hypothesis

7 0
2 years ago
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