Answer:
Hexokinase, Phosphofructokinase, Phosphoglycerate kinase and Pyruvate kinase
Explanation:
Coupled reactions are reactions that require energy by one process and is supplied by another; where the energy is transferred from one side of the reaction to the other.
In glycolysis, four (4) reactions undergo coupled reactions
1. Reaction one: which is the phosphorylation of glucose to glucose-6-phosphate coupled to the hydrolysis of ATP to ADP and Pi catalyzed by hexokinase.
2. Reaction three: The phosphorylation of fructose-6-phosphate to fructose-1-6-bisphosphate and the hydrolysis of ATP, catalyzed by phosphofructokinase
3. Reaction seven: Phosphoglycerate kinase catalyzes the conversion of Bisphoglycerate to 3-phoshoglycerate and the production of ATP from ADP
4. Reaction ten: Pyruvate kinase catalyzes the conversion of enol phosphate to the enolic form of pyruvate with the synthesis of ATP.
One major lab accident was of fire where gases were exposed to heat.
<u>Explanation:</u>
Accidents that occur in the laboratory which lead to some mishappening are known as the lab accidents. The reasons for such accidents may be many like fire, chemical spills, personal injuries and so on. To avoid all this, there should be proper check and focus while working in the lab.
One of the major lab accidents was of the fire when gas was exposed to heat. The chemical involved in this accident was the use of methane. The type of personal protective equipment worn at the time of accident was protective eye-wear and the lab coat and the lab gloves. To avoid such kind of accidents, people in lab should be very careful and it should be made sure that there are proper equipment in case of any mishap.
Answer:
May alter the specificity for its substrate
Explanation:
The active site of an enzyme refers to the specific region of an enzyme that serves as the binding site for its one or more substrates. Binding of substrates to the active site of their enzymes is required for catalysis. Enzymes are highly specific for their substrates. Type of amino acids present in the active site of the enzymes and their interactions with substrates regulate the specificity of the enzyme. If a mutation substitutes the amino acid of the active site, it may increase or decrease the specificity of the enzyme for its substrate.
