Answer: CFU/mL = 14200 CFU/mL
Explanation:
Microorganisms are very small and difficult to count directly. Instead, a laboratory technique is used to count CFUs (Colony Formation Units). This involves making dilutions of a microbial suspension and spreading it on thin layers of agar containing nutrients for growth in Petri dishes also called agar plates. After incubation under ideal conditions, some plates are completely covered with microbial growth; some have little or no growth; and some are covered with individual isolated "spots", which are microbial colonies. Since a colony may consist of a single organism or a group of microbes, the dots represent a CFU.
To calculate the viable count of the stock culture, you have to count the number of colonies, multiply by the dilution and the result will be the plate count in CFU.
So, the equation is:
CFU/mL = Number of colonies / (Volume trasferred to plate x dilution blank)
100 microliters = 0.1 mililiter
1/10 = 0.1 (dilution factor)
Replacing the information from the question:
CFU/mL = 142 / (0.1 mL x 0.1)
CFU/mL = 14200 CFU/mL
Each gene occupies a position on a chromosome<span>. Because genes provide instructions for making proteins, and proteins determine the structure and function of each cell in the body, it follows that genes are responsible for all the characteristics you inherit.</span>
In cells with a nucleus, as in eukaryotes, the cell cycle is also divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA.
