The statement saying, "<span>A demonstration speech typically revolves around a process or sequence of events</span>." is true. As the name suggests, a demonstration speech aims to inform the audience primarily through demonstrating the steps involved in accomplishing a certain task. It is then necessary that the process is done chronologically and in detail.
June 28, 1978 NASA launched it
Answer:
Disease is one and moose population.
Explanation:
Nucleus can be defined as a large organelle found in the Eukaryotic cells and it is also know as the cell's command center,thus it controls the activity of the cells
Explanation:
- The answer to the above question is (c )nucleus. During the cell cycle the nucleus of the cell mediates the replication of the of the DNA and controls the gene expression.
- The nucleus contains a structure which is known as nucleolus and which is made up of protein and ribonucleic acid(RNA)
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
