Answer:
Fredrick Douglass was a black male who spoke up for the rights of black people and the rights of women. He was extremely important to the cause of black peoples rights. I think he is an important historical contributor knowing that he did so much for black rights and even womens rights.
The Reformation began in 1517 when a German monk called Martin Luther protested about the Catholic Church. His followers became known as Protestants. Many people and governments adopted the new Protestant ideas, while others remained faithful to the Catholic Church. This led to a split in the Church.
Hopes it helps you.
The 1890 tariff was also poorly received aboard. protectionist in the British empire used it to argue for tariff retaliation and imperial preference. The tariff was not well received by Americans who suffered a steep increase in prices. In 1890 the republicans lost their majority in the house with the number of seats they won reduced nearly Halifax, from 171 to 88
Plasmids are extracellular DNA they can be present in cells but in genetic engineering you can construct them specifically add markers and restriction sites .Once constructed add the restriction enzyme which recognizes these specific restriction sites cuts it open and then you bind your gene of interest to it stick it back.
<span>So basically say your gene of interest is a blue rubber band and your plasmid is a red rubber band. Your goal is to introduce that blue band to your red band.The restriction enzyme acts like scissors cuts the red band open and maybe gives it a weird shape and then gives the same cuts to your blue band so it fits into your red band.Then you have other enzymes like ligase which basically sticks the whole think together. </span>