Answer:
it is expected that the mutation results in a reduced initiation of translation and thereby decreasing the level of the protein A, while it does not change the level of mRNA A
Explanation:
Translation in bacteria starts with the formation of the initiation complex which is composed of the small ribosomal subunit, the messenger RNA (mRNA), initiation factors and the initiator transference RNA (tRNA) containing N-formyl-methionine. The small ribosomal subunit binds to a polypurine stretch of variable length in the mRNA called 'the Shine-Dalgarno sequence'. A mutation in this sequence reduces the affinity of the ribosome for the mRNA, thereby, in this case, decreasing the level of protein A. Since transcription occurs before translation, it is expected that this mutation does not change the level of expression of the mRNA A.
Answer:
b) By mixing weak base with its conjugate acid and then adjusting the pH with strong acid.
Explanation:
Hello,
In this case, considering that the buffer required a pH greater than 7 where the basic conditions are present, we need a weak base and its conjugate base in order to assure such pH greater than 7. Moreover, by using a strong acid, we can gently add it in order to decrease the pH up to 7.4 via a properly measured neutralization of the necessary moles of base up to a hydrogen concentration of 3.98x10⁻⁸ (pH). Therefore, answer is b) By mixing weak base with its conjugate acid and then adjusting the pH with strong acid.
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