The substrate and the active site both need to fit together perfectly in order for the enzyme to work properly. If their shape is changed, typically through heat or a change in pH, the enzyme will not work.
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Answer:
The light-dependent reactions take place in the thylakoid membranes in the granum (stack of thylakoids), within the chloroplast. Figure: The two stages of photosynthesis: Photosynthesis takes place in two stages: light-dependent reactions and the Calvin cycle (light-independent reactions).
Explanation:
Each stack is called a granum (the plural is grana) which is suspended in a fluid called stroma. The light-dependent reactions occur in the grana; the light-independent reactions take place in the stroma of the chloroplasts.