Constrained by the extent of existing knowledge - Developing a hypothesis and designing an experiment is based on current human knowledge. Design of experiment is limited to observation method and instrument - e.g. discovery of viruses depended on the discovery of the electron microscope. Human error - e.g. mistakes can occur in recording observations or inaccurate use of measuring instrument.
Deliberately falsifying results - i.e. scientific fraud. Bias - prior confidence in the hypothesis being true/false can affect accuracy of observation and interpretation of results.
Explanation: biotic factors depend among abiotic factors, meaning that living things need non-living things in order to survive. That also means that biotic factors everywhere including the surrounding area need to be thought of in this sense so that they are able to survive in the environment they are in.
Hunting is regulated because plants still need to be eaten by the deer. if they arent, a plant species grows uncontrollably and kills off other plants.