When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
First the question is asking If a descision was made to drain the wetlands which a wetlands is haves rich soil and nutrients which is good for farming and an agricultural action means someone would clear out a forest of farming reasons so I would say the answer is A
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The life cycle of a goldfish starts out as an egg. The female fish will lay about 25 eggs, but unfortunately only some grow up. The eggs usually hatch within 5 days and if you have a fish tank then you should take the adult fish out or else it may eat the eggs. After the egg hatches, it turns into a fry,(this is what a baby goldfish is called) Once they hatch, they will stay attached to the plants for two days, after that, you should start to feed them. Once they are not fry anymore, they turn into adult goldfish. If you take care of them properly, they could last up to 15 years! Once the fish are complete adults, they can lay eggs. The females rub up against plants and lays eggs while the male Goldfish sprays the eggs to make them fertilize and turn into fry.
Wear safety goggles while looking into the opening of the test tube
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observe, question, research, hypothesize, experiment, test hypothesize, draw conclusions, report
