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Orlov [11]
3 years ago
10

The chromosomes arrive at the poles and nuclear envelopes form during which phase of mitosis?

Biology
1 answer:
Tpy6a [65]3 years ago
6 0

Answer:

hope this helps ♡

Explanation:

Telophase is the fifth step in mitosis. In telophase, chromosomes arrive at opposite poles and begin to decondenese. nuclear envelope material surrounds each set of chromosomes.

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One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative control organisms on
andreyandreev [35.5K]

Answer:

Reduced in holding time of decolrization step and also used less Alcohol because decolrization step is important in Gram's staining.The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative..

Explanation:

Gram' staining is a technique used in microbiology labs to differentiate between Gram's positive and negative

<u>Gram-positive bacteria :</u>Stain dark purple due to retaining the primary dye called CV in the cell wall.

<u>:Gram-negative bacteria </u>Stain red or pink due to retaining the counter staining dye called Safranin or neutral red.

There are four basic step in Gram" staining

1) Application of the Primary Stain to a Heat-Fixed Smear of Bacterial Culture

2)Addition of Gram's Iodine

3)<u>Decolorization with 95% Ethyl Alcohol:</u>Alcohol or acetone dissolves the lipid outer membrane of Gram-negative bacteria, thus leaving the peptidoglycan layer exposed and increases the porosity of the cell wall. The CV-I complex is then washed away from the thin peptidoglycan layer, leaving Gram-negative bacteria colorless.

On the other hand, alcohol has a dehydrating effect on the cell walls of Gram-positive bacteria that causes the pores of the cell wall to shrink. The CV-I complex gets tightly bound into the multi-layered, highly cross-linked Gram-positive cell wall thus staining the cells purple.

The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative. Under-decolorization occurs when the alcohol is not left on long enough to wash out the CV-I complex from the Gram-negative cells, resulting in Gram-negative bacteria to appear Gram-positive.

7 0
3 years ago
What is the answer to 4?
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Answer:Hydrolysis

Explanation:

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avier stepped on a splinter while walking barefoot. The splinter pierced deep into his foot and caused a great amount of pain. W
kykrilka [37]

Answer:

Answered below

Explanation:

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What happens to the vitamin c in a potato when it is processed into other products?
neonofarm [45]

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