A "culture boundary" is actually a word used in human geography, whereas a "political boundary" is an arbitrary line dividing one political unit.
A political boundary is a hypothetical line that divides one political unit, like a nation or state, from another. These can occasionally line up with a river or other natural geographic feature to form a boundary or other barrier between nations. Political lines frequently develop along borders between nations or states. As an illustration, the Alps divide France from Italy, while the Pyrenees form the border between France and Spain.
In reality, anthropology and human geography already use the phrase "cultural barrier." It is typically used to indicate a geographical area where a population of people shares similar cultural traits, such as language, religion, and traditions.
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Europeans carried a hidden enemy to the Indians: new diseases. Native peoples of America had no immunity to the diseases that European explorers and colonists brought with them. Diseases such as smallpox, influenza, measles, and even chicken pox proved deadly to American Indians.