Answer:
DNA digestion was not complete.
Explanation:
When a particular DNA needs to be used for the experiment, it is firstly processed by restriction digest. A restriction digest is a procedure for DNA preparation for further examination and manipulation. It is defined as a technique used for separating DNA fragments. This separation is being done using specific enzymes that will bond to specific sites and locations to the DNA so all DNA fragments will be a similar size. These enzymes are called restriction endonucleases or restriction enzymes and are extracted from bacteria for this particular cause. Once fractured or digested, DNA fragments may be amplified by PCR (polymerase chain reaction) and used for further experimentation.
When DNA is sequenced, all recognition sites (on enzymes) are visible and detectable. However, if an extra recognition site is found after sequencing, it means that initial mapping was not completed (assuming the DNA sequence has no errors). Initial restriction digestion was not completely done, so DNA is stuck in the middle of the process, in various degrees of supercoiling and digestion.
Explanation:
<h2>Hope my answer helps you</h2>
Answer:
The development of a protostomed organism begins with a spiral development. The mesoderm divides and forms the celoma. There is formation of the celloma from the blastoporo.
The development of a deuterostomy organism begins with radial and indeterminate segmentation. Archaenteron evaginations occur and celoma is formed. The anus develops from the blastoporo and the mouth from a secondary opening.
<u>Answer:</u>
<em>Repeated use of chemical fertilizers has several adverse impacts.
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<u>Explanation:</u>
<em>Chemical fertilizers are used to increase crop yield and raise agricultural productivity.</em> But a land loses its fertility over the years due to repeated fertilizer use.
<em>Excess use of fertiliser makes the soil acidicby reducing the organic content of the soil. </em>
When the amount of chemical fertilizers containing nitrogen is applied is beyond the amount that can be absorbed by the plants, it results in the release of greenhouse gases into the atmosphere.
<em>When the fertilizers are washed into water bodies their nutrient content increases heavily and this depletes the oxygen in water.
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