Mutation and variation are two simultaneous events involved in the process of evolution. The main difference between mutation and variation is that mutation is an alteration in the nucleotide sequence of a gene whereas variation is any difference between individuals of a particular species. Mutations may arise due to environmental factors such as chemicals and UV. Germline mutations are inherited by the offspring. Somatic mutations occur in the ordinary body cells. Variation can occur due to mutations, genetic recombination, gene flow, genetic drift, random mating, random fertilization, and environmental factors. Variation may also cause evolution through natural selection.
when something causes the enzyme to change shape, thus changing shape of the active site and not allowing any substrate molecules to fit in the site and bind. Denaturation makes it so reactions don't occur between the substrate and the enzyme