Answer:
Cut open the plasmid and "paste" in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).
Insert the plasmid into bacteria. Use antibiotic selection to identify the bacteria that took up the plasmid.
Grow up lots of plasmid-carrying bacteria and use them as "factories" to make the protein. Harvest the protein from the bacteria and purify it.
Explanation:
It would be renamed homeless lizards.
Largest energy found in the lowermost portion of the trophic level near "Producers" 'cause as it passes through the higher trophic levels, it get decreased by 10% at each step, according to Lindmann's 10% law!!
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Answer:
a. to prevent the unnecessary breakdown of glycogen
b. to prevent the formation of glucose
e. to prevent hydrolytic cleavage of glucose 1‑phosphate
Explanation:
the main reason is to prevent hydrolysis. because the entry of water could lead to the formation of glucose rather than glucose 1-phosphate.
When every dilution of your sample results in a plate that is too few to count we should filter the original sample and use most probable number method .
Probable number method count and determine the number of bacteria in a culture, plate aliquots of the dilutions onto agar with sterile pipettes, spread with glass sticks, incubate at 37°C and count the number of colonies. It is imperative that you utilize your best culture technique.
Not all bacterial cells produce colonies, as some bacteria tend to clump or aggregate, and some are nonviable. For this reason results are reported as colony forming units (CFU)/ml of bacterial culture. Ideally only plates with 25-250 colonies are used.
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