Answer:
- Bacteria cannot remove intronic sequence from a gene, so if the gene for factor VIII were transcribed, it would translate to a nonfunctional protein.
- The bacteria will not recognize the eukaryotic promoter to transcribe the gene.
Genome engineering steps:
I. Isolate by PCR the mature mRNA of the target factor VIII gene
II. Synthesize a complementary DNA (cDNA) for this mRNA by using a reverse transcriptase enzyme
III. Insertion of the cDNA into a plasmid vector
IV. Expression of the protein in the host bacterial cell through transformation
Explanation:
Bacteria lack the spliceosome (ie., the eukaryotic machinery required to remove introns from the primary mRNA), because bacterial genes do not have introns. Moreover, eukaryotic organisms require several proteins that bind to the promoter sequence and subsequently recruit the RNA polymerase to initiate this process. In bacteria, transcription is controlled by proteins that bind to cis-acting sequences which regulate the transcription of adjacent genes. In contrast to bacteria, eukaryotic genes have many classes of promoter and enhancer elements.
Genome engineering technologies can be used to produce bacteria expressing human proteins derived from synthetically introduced human genes (see steps above). For example, recombinant bacteria expressing the human insulin gene have already been designed by using E. coli as cell factories.
Answer:
The correct answer is C) 8 exons.
Explanation:
If we assume that each domain matches with an exon, and we have a 6 domain protein (domain= the functional regions of the protein that include the active site), there must be at least 6 exons in the gene. Also, remember that exons include the 5'- and 3'- untranslated regions or UTR. So, assuming that each unstraslated region is equivalent to an exon, there must be at least 8 exons in the gene (6 exon for each domain and one 2 exons for UTR).
Anyway, be careful, because this is a simple exercise where we assume a lot of things. In the real world, the first exon tends to include the 5'-UTR and it is already proved that one exon is not always equivalent to one protein domain.
Answer:
The relative age of a rock then is its age in comparison with other rocks. If you know the relative ages of two rock layers, (1) Do you know ...
Explanation:
The answer is (A). Hope this helps
The answer is C) Dripstone produced by a buildup of minerals precipitated fro. groundwater.
