This affected slaves in a negative way because since the fugitive slaves would be returned to their owner, the slaves would later suffer punishment, beatings, and even death due to them escaping their master. It was a terrible agreement that no state could stop a fugitive slave from being returned to his/her owner.
By discovering a synthetic cell . I hope you have a great day
I would say D! Purely because it is looking at the effects of humans (therefore related to topics more geographical).
Hope that helps. :)