Question

If the mutation occurred where one base was changed to another within the cutting area of a restriction enzyme what would happen?

Answer 1

The base change in the mutation of the cutting area of a restriction enzyme would determine whether a person is the bearer of that particular DNA.  

Explanation:

The digestion of nucleic acid is done by the enzymes of restriction. It helps in recognizing the nucleotides sequence inside a DNA strand.  It’s use detects in a DNA the points of mutation and helps in eliminating the need of sequencing.

This enzyme cuts DNA which later can be seen by using electrophoresis gel the bearing of the sequence by an individual. But this enzyme won’t recognize the site if any nucleotide pair is replaced.