Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
They will have a large amount of genetic variation.
Explanation:
Scrapping pollens obtained from one plant to the flower of another plant (different variety but same species) would lead to increase in the genetic pool. The new hybrid plant would carry genetic features from both plants varieties. This process is also used to develop new varieties and have been recognized as cross-pollination. This process is generally preferred over genetic modification (GM) because of more nature friendly.
Oh man i remember learning about this but i cant remember what the answer is i will see what I can do :D
For those actually in relationships, shyness was negatively associated with intimacy satisfaction. Shyness was also positively associated with insecure romantic attachments, including greater attachment anxiety and attachment avoidance. For males especially, shyness also had a negative relationship with overall psychological well-being.
