The barrier is cultural differences.
John, growing up in America learned that eye contact is a way of respect and to show you are paying attention.
Where Cho, growing up in Asia, many Asian counties don't have this "custom" to call it that.
Answer: Gene alterations in gametes
Explanation:
The vacuole is a "container" in the cytoplasm in a cell that stores water, food, and waste.
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http://www1.lsbu.ac.uk/water/cellulose.html