typical, well aerated culture will have a density of about 109 or 1010 cfu/mL after an overnight incubation. If you are working
1 answer:
We have that the dilution factor to get 30-300 colonies is mathematically given as
dilution factor=10^8
<h3>Dilution factor</h3>
Question Parameters:
A density of about 109 or 1010 cfu/mL.
plate that culture to get 30-300 colonies on your plate.
Generally, Concentration of cells in the culture
C= 2x10^10 cfu/mL
Let's goal 200 colonies.
Let's extent plated be 1 mL
2e10 = 200\times dilution
dilution factor=
dilution factor=10^8
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Complete Question
A typical, well-aerated culture will have a density of about 10^9 or 10^10 cfu/mL after an overnight incubation. If you are working with a strain of bacteria that grows to 2x10^10 cfu/mL after overnight incubation, how should you dilute and plate that culture to get 30-300 colonies on your plate? What dilution should you use?
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