Question

typical, well aerated culture will have a density of about 109 or 1010 cfu/mL after an overnight incubation. If you are working with a strain of bacteria grows to 2x1010 cfu/mL after overnight incubation, how should you dilute and plate that culture to get 30-300 colonies on your plate

Answer 1

We have that the dilution factor to get 30-300 colonies is mathematically given as

dilution factor=10^8

Dilution factor

Question Parameters:

A density of about 109 or 1010 cfu/mL.

plate that culture to get 30-300 colonies on your plate.

Generally, Concentration of cells in the culture

C= 2x10^10 cfu/mL

Let's goal 200 colonies.

Let's  extent plated be 1 mL

$C= \frac{(number of colonies \times dilution factor)}{volume plated }$

2e10 = 200\times dilution

dilution factor= $\frac{2x10^10}{200}$

dilution factor=10^8

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Complete Question

A typical, well-aerated culture will have a density of about 10^9 or 10^10 cfu/mL after an overnight incubation. If you are working with a strain of bacteria that grows to 2x10^10 cfu/mL after overnight incubation, how should you dilute and plate that culture to get 30-300 colonies on your plate? What dilution should you use?