It is A , d , and c . I can bet my last bagel bite
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
The (rough) endoplasmic reticulum
Explanation:
The endoplasmic reticulum is a system of interconnected membranes that functions in the synthesis of several membrane-related proteins and lipids. There are two types of endoplasmic reticulum in the eukaryotic cell;
- Smooth endoplasmic reticulum
- Rough endoplasmic reticulum
The smooth endoplasmic reticulum is actually smooth because it has no ribosome attachment while the rough endoplasmic reticulum appears rough due to the attachment by ribosomes.
<em>Therefore, the name of the specific organelle that is studded with ribosomes in eukaryotic cell is endoplasmic reticulum.</em>
Answer:
Recessive
Explanation:
When there is a skipped generation, the trait must be RECESSIVE. For dominant trait, having 1 dominant allele will give you affected phenotype. It can never give a skipped generation.
