There could be a lot of factors that could be a hinder towards a successful escape and these factors may include: The barrier or shield in the facility in which this could be a problem if you don't have the clue of where you're getting out of, the tools that you are going to used can be also one of the problems because you won't know what more tools you need in order for escape and lastly, people you're with or the people who has also been held captive can be a factor because you won't know who to trust.
1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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For Kohlberg, the Post-Conventional individual is the one who follows their own rules by their own ethical principals. This individuals also think that not all rules must be followed without question. Some theorists think that many people may never reach this level and I agree. Society makes us be influenced by other’s people’s opinions, so it is very hard for an individual to not care about the public perception of themselves, I included.
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Explanation:
we can get the information about past by studying and collecting information about past and using the sources like manuscripts , inscriptions and archaeology.
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Answer:
I think this will help....i didnt wanna give the actually awnser so here
Explanation:
The Ghana Empire (c. 700 until c. 1240), properly known as Wagadou (Ghana or Ga'na being the title of its ruler), was a West African empire located in the area of present-day southeastern Mauritania and western Mali. Complex societies based on trans-Saharan trade in salt and gold had existed in the region since ancient times,[1] but the introduction of the camel to the western Sahara in the 3rd century CE, opened the way to great changes in the area that became the Ghana Empire. By the time of the Muslim conquest of North Africa in the 7th century the camel had changed the ancient, more irregular trade routes into a trade network running from Morocco to the Niger river. The Ghana Empire grew rich from this increased trans-Saharan trade in gold and salt, allowing for larger urban centres to develop. The traffic furthermore encouraged territorial expansion to gain control over the different trade routes.
