Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Interphase, the first part of the cell cycle
A. 1.9 mL to 8.7 mL because 1.9 is the lowest and 8.7 is the highest and the range is from lowest to highest.
Answer: a. rna interference.
Rna interference <span> was known by other
names like <span>co-suppression because
it </span>has become evident that RNAi has immense potential in inhibiting desired
genes by neutralizing targeted mRNA molecules</span> Two types of small ribonucleic acid (RNA) molecules that are central to RNA interference
are miRNA and <span>siRNA. </span>
A snowflake forms when water vapor<span> sublimates, or </span>turns directly<span> from a gas </span>into <span>its </span>solid form<span>, </span>ice<span>.</span>
