The best way may be is going over the notes on the chapter you were doing. Or going over the chapter once or twice.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Cory is reluctant to leave her home and attend college despite just being finished with her teenage years. Usually, the people of this age are explorative and are trying to find their identity in the world separately, but Cory seems to stay isolated even from Mike, which indicates that she might be going through the stage of stress. Stress makes a person to avoid any kind of interaction with other people and inhibits the urge to explore the life outside one's comfort zone. So, Cory is going through the stress stage.

<u>Aerobic </u><u>respiration </u>:- Aerobic respiration is process of respiration in which complete oxidation of glucose takes place in the presence of oxygen
<u>That </u><u>is</u><u>, </u>
<u>Aerobic </u><u>respiration </u><u>is </u><u>takes </u><u>place </u><u>in </u><u>four </u><u>stages </u><u>:</u><u>-</u>
- <u>Glycolysis</u>
- <u>The </u><u>link </u><u>reaction </u>
- <u>The </u><u>Krebs </u><u>cycle </u>
- <u>Oxidative </u><u>phosphorylation </u>
In aerobic respiration, Glucose with 6 carbon atoms broken into 2 pyruvic acid each with 3 carbon atom and produces water + CO2 + energy.
<h3><u>Whereas</u><u>, </u></h3>
<u>Anaerobic </u><u>respiration </u>:- is the process of respiration in which incomplete oxidation of glucose takes place in the absence of oxygen
The end products of glucose in anaerobic respiration is
- <u>In </u><u>human </u><u>muscles</u><u>, </u><u> </u><u>Lactic </u><u>acid </u><u>+</u><u> </u><u>Energy </u>
- <u>In </u><u>yeast</u><u>, </u><u>Ethanol </u><u>+</u><u> </u><u>CO2</u><u> </u><u>+</u><u> </u><u>Energy </u>
<u>Hence</u><u>, </u><u>Option </u><u>D </u><u>is </u><u>the </u><u>correct </u><u>answer</u><u>.</u>
It Is called, a mirror image or reflection.