Answer:
The plasmid must express a gene for ampicillin resistance (the protein product of the <em>bla</em> gene codes for beta-lactamase, the protein that breaks down ampicillin). The colonies on the ampicillin plate are antibiotic resistant. This means that they have taken up the transformed plasmids expressing both the <em>bla</em> gene and the GFP gene.
Explanation:
The transformation involved the genetic modification of a plasmid to incorporate the gene encoding the green fluorescent protein (GFP) from jelly fish. GFP makes cells glow under UV light.
In genetic engineering, scientists use antibiotic resistance as markers to indicate cells that have been transformed. By incorporating an antibiotic resistance gene such as <em>bla</em> into the vector (plasmid) and then growing the cells in antibiotic media, scientists determine which colonies have taken up the plasmid. Therefore, if the cells survive, this means that they contain the plasmid with antibiotic resistance gene as well as the GFP gene.
No, loses less water than usually than think
NADPH is the reduced form of NADP+. This simply means that NADPH has an extra hydrogen ion.
NADPH is formed when upon the reduction of NADP+ . NADP stands for nicotine amide dinucleotide phosphate. It is a coenzyme that is involved in a wide range of anabolic reactions.
During the synthesis of carbohydrates in plants, NADPH acts as a reducing agent, likewise in the synthesis of nucleic acids and lipids.
As a reduced compound, NADPH becomes a molecule with high energy thus being especially useful in driving the Calvin cycle.
The physical processes on Earth create constant change. These processes—including movement in the tectonic plates in the crust, wind and water erosion, and deposition—shape features on Earth's surface.
